Oral Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Towards clinical O-glycomics (67183)

Niclas Karlsson 1
  1. Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden

The incidence of osteoarthritis is increasing to almost epidemic levels due to an aging population in developed countries. Our group is committed to approach osteoarthritis from an O-linked glycobiological angel. By doing this we have developed and adopted tools for O-glycodiscovery and clinical O-glycomics. The O-glycome and O-glycoproteome of synovial fluid from osteoarthritic patient was revealed using MS. Pathological changes, including sulfation pattern of the O-linked glycome was identified. In addition to standard CID, we adopted Ion-Mobility MS (IM-MS) and Selected Reaction Monitoring (SRM) to into identify sites of sulfation of core-1 structures Gal1-3GalNAc with or without sialic acid. This included sulfation of non-reducing terminal galactose and sialic acid as well as reducing terminal GalNAc residues. We then used recombinant expression of glycosyltransferases in CHO-cells to identify glycosyl- and sulfo-transferases responsible for modification of the osteoarthritic glycome. Finally, we demonstrated the power of SRM for screening of patients sample and compared it to a developed lectin-ELISA for OA diagniostics that could be used in clinics.