Oral Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Multidimensional proteomic study identifies decreased protein synthesis and increased histone 2A ubiquitylation during aging (#9)

Lu Yang 1 , Zaijun Ma 1 , Nan Liu 1 , Yaoyang Zhang 1
  1. Interdisciplinary Research Center on Biology and Chemistry, Chinese Academy of Sciences, Shanghai, SH, China

The long-lived proteome constitutes a pool of exceptionally stable proteins with limited turnover. Previous studies on ubiquitin-mediated protein degradation primarily focused on relatively short-lived proteins; how ubiquitylation modifies the long-lived proteome and its regulatory effect on adult lifespan is unclear. Here we profile the age-dependent dynamics of long-lived proteomes in Drosophila by mass spectrometry using stable isotope switching coupled with antibody-enriched ubiquitylome analysis. Our data describe landscapes of long-lived proteins in somatic and reproductive tissues of Drosophila during adult lifespan, and reveal a preferential ubiquitylation of older long-lived proteins. We further used pulsed metabolic stable isotope labeling analysis to characterize protein synthesis during aging. Interestingly, this study determines an age-modulated decline in protein synthesis with age, particularly in the pathways related to mitochondria, neurotransmission, and proteostasis. Importantly, we identify an age-modulated increase of ubiquitylation on long-lived histone 2A protein in Drosophila, which is evolutionarily conserved in mouse, monkey, and human. A reduction of ubiquitylated histone 2A in mutant flies is associated with longevity and healthy lifespan. Together, our data reveal proteomic dynamics during aging and an evolutionarily conserved biomarker of aging that links epigenetic modulation of the long-lived histone protein to lifespan.