Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Plasma proteome profiling for the discovery of predictive biomarkers to identify Neurological Heterotopic Ossification (NHO) (#506)

Mriga Dutt 1 , Hsu-Wen Tseng 2 , Kylie Alexander 2 , Jean-Pierre Levesque 2 , Michelle Hill 1
  1. Precision and Systems Biomedicine, QIMR Berghofer MRI, Brisbane, QLD, Australia
  2. Mater Research, The University of Queensland, Brisbane, QLD, Australia

Neurological Heterotopic Ossification (NHO) is a severe trauma-induced condition where extra-skeletal bone mineralisation occurs in soft tissues. NHO is a frequent complication of traumatic brain injury (TBI) or spinal cord injury (SCI) and often develops around joints, which causes chronic pain and joint deformation in patients; reducing the patient's quality of life. The mechanisms leading to NHO are unknown and the only effective treatment remains surgical resection. To elucidate NHO pathophysiology, we developed the first NHO model in genetically unmodified mice, which mimics most clinical features of NHO. In order to identify novel predictive biomarkers of NHO, plasma was collected from mice developing NHO. Depleted plasma from control and NHO mice (n=10 per group) was subjected to label-free, bottom-up tandem mass spectrometry on the SCIEX TripleTOF 5600, and subsequent protein similarity search and statistical analyses were performed using MaxQuant v1.5.8.0 and Perseus v1.6.2.3 respectively. Overall, 160 proteins were retrieved and of these, 13 proteins were identified to be differentially expressed between control and NHO cohorts. Extracellular matrix protein-1 (ECM1) and vitronectin (VTNC) appeared to be down-regulated in the NHO cohort, suggesting their putative role in triggering early NHO formation. These findings however, need to be validated in future studies. In conclusion, we have characterised the plasma proteome of an SCI mouse model to identify putative predictive biomarkers inducing the onset of NHO development, following a spinal-cord injury.