Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Proteome survey of wound fluid from non-healing wounds reveals key biological processes associated with poor healing outcomes (#444)

Daniel A Broszczak 1 2 , James A Broadbent 1 2 , Tony J Parker 1 2
  1. School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia
  2. Tissue Repair and Translational Physiology, Institute of Health and Biomedical Innovation, Kelvin Grove, Queensland, Australia

Non-healing wounds are a significant problem for patients and healthcare systems worldwide. The underlying biochemistry, which drives non-healing outcomes in self-perpetuating leg wounds, is poorly understood. To address this knowledge deficit, a study of the proteins that compose the fluid, which exudates from these wounds, may provide important insight regarding treatment response and healing outcome for patients. In this respect, we have conducted a clinical study that included the collection of biological samples and clinical / psychosocial data over a 24 week period, during which time patients received best-practice care. Biological samples were analysed using data-independent acquisition SWATH-MS to identify and quantify the protein complement of the wound fluid. The resulting data were integrated with clinical measurements and contextualized by gene ontology annotations to enable deeper insight into the dynamic biological processes taking place within non-healing wounds. This identified key biological processes that may indicate specific underlying issues for a sub-set of wounds and their recalcitrant nature towards clinical care. A number of biological markers that are indicative of the wound healing outcome were also derived from these analyses. Unravelling the complex biology of non-healing wounds through proteome and clinical data integration provides some insight into the mechanisms associated with a patient’s adverse or positive responses to clinical care. Such information can be developed further to inform clinical practices and enable the meaningful personalisation of wound management.