Radiation therapy show various cellular responses to Human lymphocytes.However, the molecular mechanisms of radiation-induced cell differentiation, malignant transformation, and apoptosis are not fully understood. In this study, changes in protein in B-lymphocyte (GM10832) in various radiation exposure environments were LFQ (Lable Free Quantification) analyzed by LC-ESI-MS / MS and Proteome Discoverer software. As a result, we were able to identify protein groups that vary with irradiation dose and protein groups that change with time after irradiation. In addition, T acute lymphoblastic leukemia cells (Molt4) were used to find protein groups induced by radiation in cancer cells. Identification of proteins for cellular responses will potentially provide deeper insights into radiation-induced cytotoxicity, particularly interesting in radiation therapy and protection programs.