Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Analysis of proteome and phosphoproteome in response to higher temperatures in rice cultivars (#410)

Dalal Alonazy 1 , Ramesh Katam 1 , Peter Scott 2 , Sara Bergin 3 4 , Raja K Reddy 5
  1. Department of Biological Sciences, Florida A&M University, Tallahassee, Florida
  2. University of Florida , Gainesville, FL, US
  3. Biological Sciences , Florida A&M University , Tallahassee, Florida, US
  4. Des Moines Area Community College, Ankeny, IA, USA
  5. Mississippi State University, MS, Mississippi State, USA

Rice is the third most important agricultural commodity worldwide. Annual increase in the global temperature has an adverse effect on the crop productivity and nutritional quality. During the temperature stress, plants undergo various physiological, biochemical changes occur in the plant to adapt to high temperatures levels. The present study was aimed to investigate the relative changes in proteome and phosphoproteome in response to higher temperatures in rice cultivars. The specific objectives are to: (i) characterize of heat stress responsive proteins in tolerant and susceptible rice cultivars (ii) determine phosphorylated protein modifications in response to heat stress (iii) evaluate the proteins associated with heat tolerance mechanism and their biosynthetic pathways. Heat tolerant (Cv-5) and susceptible (Cv-13) rice cultivars were used to investigate the changes in proteome changes to heat stress in rice panicle. Plants were treated with three temperature levels; i) Optimum control 28/20°C, day/night, ii) Moderate32/24°C, and iii) high 36/28°C. Plant growth and yield-related parameters were determined after 137 days sowing till harvest. Proteins were extracted from frozen panicle tissue and iTRAQ labeled, following hybrid quadrupole-TOF QSTAR Elite MS/MS system and TripleTOF™ 5600. A total of 779 proteins were identified in control and treated samples. Twenty-seven proteins found to be phosphoproteins. When compared to the proteins responded to temperature, Cv-5 showed the abundance of highest number of proteins in response to higher temperature treatments. Gene ontology enrichment analysis with agriGO indicated the majority of the identified functions include response to abiotic stimulus, metabolites. Cv-5 displayed highest amount of proteins that are associated with defense responses and environmental stresses. The proteome data was validated with the expression levels of transcripts. This study provides new insight on proteins and phosphoproteins contribute to the temperature tolerance.