Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Novel Method for Proteome-scale Analysis of Protein Substrates and Modification Sites of Small Ubiquitin-like Modifier (#835)

Yichu Shan 1 , Yang Li 1 , Lihua Zhang 1
  1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, LIAONING, China

Small Ubiquitin-like Modifier(SUMO) plays important roles in regulating many physiological activities of cells. Accurate identification and quantification of the SUMO substrates and modification sites at proteome scale is helpful for studying the molecular mechanisms of SUMO modification and its' influence on the functions of protein substrates. However, there is no method for the selective enrichment and efficient and accurate identification of wild-type SUMO modified peptides in various kinds of protein samples. In this work, a method for enrichment of endogenous SUMOlyated peptides based on anion chromatography and antibody was developed. Standard SUMO modified peptides library was synthesized and high efficient and accurate algorithms for the identification of SUMO modified peptides was developed by investigating their fragmentation patterns in mass spectrometer. The developed method was applied to the proteome-scale analysis of SUMO modified proteins and modification sites to the analysis of SUMO substrates and their modification sites in HeLa cells, which provides important technical support for disclosing the regulation networks of SUMO modification and studying the functions of SUMO modification in biological processes.