The proteomic research at present is crippled in certain sense, as it focuses on analyzing thousands of proteins over a few samples. An equally important part of proteomic research where a few proteins are examined over thousands of samples (quantitative proteomics), is missing. We introduced here Quantitative Dot Blot (QDB) analysis, an immunoassaywhich can achieve absolute quantitation of a specific protein over thousands of samples in high throughput format. The application of this technique in clinical diagnosis has achieved unprecedented success, where the subtyping of breast cancer patients, which relies traditionally on genetic analysis, is realized with the 3D distribution of three key protein biomarkers of Estrogen receptor (ER), Progesterone Receptor (PR) and Her2 as absolute and continuous variables. Continuing research on quantitative proteomics should have significant impact on the practical side of precision medicine, and it would also complement the current proteomic research to present a whole picture of proteomics in the future.