Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

A rapid and highly selective method for N-glycopeptide enrichment by bacterial cellulose (#923)

Mengxi Wu 1 , Weiqian Cao 1 , Xinwen Zhou 1 , Siyuan Kong 1 , Pengyuan Yang 1
  1. Fudan University, Shanghai, China

Glycosylation is considered as one of the most frequent post translational modifications, which has great influence on many biological processes. Glycopeptide enrichment with high efficiency is critical in glycosylation research. However, most of the current enrichment methods of N-glycosylated peptides are low efficient, expensive and labor-consuming. Herein, we present a cost-friendly, biodegradable and easily obtainable material, bacterial cellulose, to enrich intact N-glycopeptides from different samples. The enrichment method based on this material turned out to be highly selective, easy to operate, and can maintain the intact glycopeptides. With this materials, intact glycopeptides can be easily obtained through incubation of tryptic digested peptide mixtures with a small piece of bacterial cellulose for merely 10 minutes. With this method, we successfully identified 97 intact N-glycopeptides from human IgG proteins, and 650 intact N-glycopeptides from human serum samples.  In addition, the enrichment specificity of this method is more than 80%. Overall, the enrichment method developed in this study shows good performance compared with other existed methods, and a great potential in further application.