Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Differential protein expression in Pelistega indica under anaerobic vs aerobic conditions (#845)

Om Prakash 1 , Roger Mercer 1 , Rakesh Singh 1
  1. Florida State University, Tallahassee, FLORIDA, United States

Recent studies related to human gut microbiota have revealed the direct or indirect role of gut microbes in human health and disease. Gut microbes are involved in various activities such as nutrient metabolism, degradation of xenobiotics and drugs, immunomodulation, prevention of pathogen invasion and maintenance of gut mucosal barrier. Pelistega indica is a Gram negative, non-spore-forming, facultative anaerobe isolated from human fecal material. We conducted comparative proteomic study of Pelistega indica grown under aerobic and anaerobic conditions on Q-Exactive HF. Whole cell protein was extracted from cultured bacteria and the proteome was analysed using nLC-MS/MS. We detected more than 1200 proteins with high confidence. The data will be presented indicating significant differences in specific pathways between aerobic and anaerobic conditions.