Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

propro: a web-based and cloud computing platform for Data-Independent Acquisition Mass Spectrometry Data Interpretation and Visualization (#704)

Miaoshan Lu 1 , Ruimin Wang 1 , Shaowei An 1 , Tiannan Guo 1 , Changbin Yu 1
  1. Westlake University, Hangzhou, NOT US OR CANADA, China

Data-independent acquisition (DIA) mass spectrometry is gaining popularity in proteomics research however the data interpretation and visualization are limiting its application and further development due to the huge data amount and the requirement of heavy computing.

Here, we developed a novel one-stop cloud platform for convenient DIA data interpretation and visualization which can be deployed on both private and public cloud servers. The advancement of propro over existing software tools include: 1) a novel data format "Aird" with up to 89%-95% compression ratio; 2) experiment analysis with a 420000 target peptides library only cost ~15 minutes by using a personal computer (CPU: i7 7700 4.2GHz, 16G memory, HDD Disk) but with up to ~20% more peptide identifications;  3) Graphic interfaces for every intermediate steps which enable users to perform the entire workflow transparently; 4) availability of OpenAPI for community-based further development of the software; 5) also allows effective analysis and visualization of parallel reaction monitoring (PRM) data sets.

In conclusion, propro is a novel and comprehensive cloud platform for Interpretation and visualizing DIA data sets. It is available at Github:  Everyone can visit propro on