Oral Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Proteomics of Extracellular Matrix Remodeling Following Myocardial Infarction (#91)

Merry L Lindsey 1
  1. University of Nebraska Medical Center, Omaha, NE, United States

Following myocardial infarction (MI), the left ventricle (LV) undergoes a series of cardiac wound healing responses that involve stimulation of robust inflammation to clear necrotic myocytes and tissue debris and induction of extracellular matrix (ECM) protein synthesis to generate a scar. Proteomic strategies provide us with a means to index the ECM proteins expressed in the LV, quantify levels, determine molecular and cellular physiology, and explore interactions. This talk will focus on the major cell types that coordinate cardiac wound healing, namely the infiltrating leukocytes and the cardiac fibroblasts. We will discuss efforts in proteomics research that have expanded our understanding of post-MI LV remodeling, concentrating on the strengths and limitations of different proteomic approaches to glean information that is specific to ECM turnover in the post-MI setting. We will discuss how recent advances in sample preparation and labeling protocols increase our successes at identifying components of the cardiac ECM proteome. We will summarize how proteomic approaches, focusing on the ECM compartment, have progressed over time to current gel-free methods using decellularized fractions or labeling strategies. In summary, this talk will provide an overview of how cardiac ECM proteomics has evolved over the last decade and will provide insight into future directions that will drive our understanding of cardiac ECM turnover in the post-MI LV.