CKD is a disease clinically defined as the patients have kidney function loss and insidiously reached to the end-stage kidney failure, in which they need to be treated with dialysis therapy or renal transplantation. Since causes of CKD are various, CKD is pathologically classified into several disease types including diabetes, hypertension, chronic glomerulonephritis, polycystic kidney disease and so on by histological examination of kidney biopsy. However, in spite of the pathological diagnosis, specific therapies for each type have not been developed as the pathological mechanisms of CKD or each CKD type have not been elucidated yet.
To understand the pathological mechanisms of each CKD type more precisely, we aimed to analyze CKD kidney biopsy specimens by proteomics. Firstly we developed a method to obtain peptides of enough amounts (~1