Submitter Withdrawn HUPO 2019 - 18th Human Proteome Organization World Congress

Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy   (#843)

Margaret Simonian 1 2 3 , Dyna Shirasaki 1 , Vivienne Lee 2 , David Bervini 4 , Michael Grace 5 , Rachel Ogorzalek Loo 1 , Joseph Loo 1 , Mark Molloy 6 , Marcus Stoodley 2
  1. UCLA, Los Angeles, CALIFORNIA, United States
  2. Department of Clinical Medicine, Faculty of Medicine and Health Sciences, , Macquarie University,, Sydney,, NSW, , Australia.
  3. David Geffen school of medicine, UCLA, Los Angeles, CA, United States
  4. Neurosurgery Department,, Bern University Hospital,, Bern,, Switzerland
  5. Genesis Cancer Care, , Macquarie University Hospital , Sydney, , NSW,, Australia
  6. Lawrence Penn Chair of Bowel Cancer Research,, Faculty of Medicine and Health, Northern Clinical School, Sydney, , NSW, Australia

Abstract

Background

Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. This aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.

Methods

We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MSE) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach.

 

Results

Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. Profilin-1, ESM-1, ion channel proteins, Annexin A2 and lumican.

Conclusion

 This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery

Key words

In vivo biotinylation, AVM molecular therapy, membrane proteins, radiosurgery