Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Comparative proteomic analysis reveals cellular response to ionizing radiation exposure in bone marrow of mice (#940)

Jeonghun 1 Yeom 1 , Jiyoung 1 Yu 1 , Hee Sung 1 Ahn 1 , Hwangkyo 1 Jeong 1 , Je-Won 1 Ryu 1 , Sang-wook 1 Lee 2 , Kyunggon 1 Kim 1
  1. Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea
  2. Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, SEOUL, Republic of Korea

Radiation exposure such as radiation therapy inevitably causes side effects in normal tissues and cells. These side effects are typically inflammatory reactions due to abnormal bone marrow responses. In order to understand the molecular mechanism of these side effects, we tried to identify the differences between Lipopolysaccharide(LPS)-induced sepsis model mice and radiation exposed mice. Sepsis model mice except normal mice and radiation exposed mice were also analyzed by considering the conditions for 4 hours and 24 hours after treatment to observe the change with time. For proteomics analysis, mouse bone marrow of each condition was extracted and mass analysis was performed by obtaining protein from the extracted bone marrow. A TMT-labeling quantification method was introduced for comparative analysis of each sample. A total of 4341 proteins were excavated through three repeated analyzes. Among them, 2,675 proteins containing quantitative information in each sample were used for comparative analysis. As a result, it was confirmed that the sample was exposed to radiation rather than the sample of other conditions, and the change of the proteome of the sample after 24 hours appeared. In particular, we observed changes in NRF2-mediated oxidative stress response. These results will help to understand the mechanism of the inflammatory response caused by radiation exposure.