Poster Presentation HUPO 2019 - 18th Human Proteome Organization World Congress

Immunoproteomics approach to discover immunogenic SFTS virus antigen for vaccine and diagnostic kit development  (#945)

Jiyoung Yu 1 , Eunsil Kim 2 , Hee-Sung Ahn 1 , Jeonghun Yeom 1 , Yumi Oh 1 , Hwangkyo Jeong 1 , Yujin Jo 1 , Keun Hwa Lee 3 , Yangsoo Kim 2 , Kyunggon Kim 4
  1. Dept. of Convergence Medicine, Asan Medical Center, Songpa-gu, SEOUL, South Korea
  2. Dept. of Infectious Disease, Asan Medical Center, Seoul, South Korea
  3. Dept. of Microbiology and Immonolgy, Jeju National University, Jeju, South Korea
  4. Dept. of Convergence Medicine, Asan Medical Center/University of Ulsan, Seoul, South Korea

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease in Asia area from 2013. The major clinical symptoms of SFTS are fever, vomiting, diarrhea, multiple organ failure, thrombocytopenia , leucopenia and elevated liver enzyme levels, showing its fatality rates ranging from 12% to as high as 30%. SFTS virus is a phlebovirus in the family of Bunyaviridae, and consist of 3 gene segments, large (L), medium (M) and small (S). And 6 proteins have been identified—an RNA dependent RNA polymerase (RdRp), a glycoprotein precursor (M), a glycoprotein N (Gn), a glycoprotein C (Gc), a nuclear protein (NP) and a non structural protein (NSs). In this study, using immunoproteomics approach coupled with high resolution LC-MS platform, several highly immunogenic SFTSv antigens were discovered. Those antigens are expected to be applied in vaccine development and rapid detection kit development. Also, immunoproteomic approach shows its possibility as a useful tool for antigen discovery.